What does MitoTracker deep red measure?

MitoTracker Deep Red (MTDR) is a relatively non-toxic, carbocyanine-based, far-red, fluorescent probe that is routinely used to chemically mark and visualize mitochondria in living cells. Dhuʻl-H. 20, 1442 AH

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In respect to this, how is mitochondrial dysfunction measured?

Measurement of the oxygen consumption rate (OCR) Oxygen consumption is an excellent read-out for mitochondrial respiratory activity. Measurement of the oxygen consumption rate (OCR) is the current experiment of choice to determine underlying mitochondrial dysfunction [36, 37]. Correspondingly, what does mitotracker red bind to? However, MitoTracker is chemically reactive, linking to thiol groups in the mitochondria. The dye becomes permanently bound to the mitochondria, and thus remains after the cell dies or is fixed. In addition, it can be used in experiments in which multiple labeling diminishes mitochondrial function.

Correspondingly, can mitosox be fixed?

As far as I know, MitoSOX is not fixable. Hey Bhabesh Kumar Tripathy , the uptake of MitoSOX into the mitochondria is dependent on the mitochondrial membrane potential. When there is no membrane potential anymore, MitoSOX will leak out. Moreover, who are mito and tracker? The initial implantation of two embryos resulted in the birth of healthy twin monkeys, nicknamed “Mito” and “Tracker” (in reference to the procedure used for imaging of mitochondria). These monkeys are the world's first animals derived by spindle transfer.

Thereof, is mitotracker deep red dependent on membrane potential?

Our data and previous reports suggest that MitoTracker Deep Red is a mitochondrial potential-dependent dye and therefore should not be used to quantitatively assess mitophagy.